Recombinant
RabMAb

Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)

Overview

  • Product name
    Anti-CD18 (phospho S745) antibody [EP1288Y]
    See all CD18 primary antibodies
  • Description
    Rabbit monoclonal [EP1288Y] to CD18 (phospho S745)
  • Host species
    Rabbit
  • Specificity
    This antibody only detects CD18 phosphorylated on Serine 745.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human CD18.

  • Positive control
    • Jurkat cell lysate or human tonsil tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: Raw 246.7
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.
    • Involvement in disease
      Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications
      Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    Images

    • ab52920 (purified) at 1:50 dilution (2µg) immunoprecipitating CD18 in Jurkat whole cell lysate.
      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab52920 & Jurkat whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution (unpurified)

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with AP.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 85 kDa
      Observed band size: 85 kDa

    References

    ab52920 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihre Email.

    Beide Nummern (1213063 und GR31039-4) beschreiben dasselbe Lot.

    Ich kann bestätigen, dass die Konzentration für dieses Lot 1.283 mg/mL ist.

    ich hoffe, diese Information ist hilfre...

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    Vielen Dank für Ihren Anruf.

    Ich freue mich nun die Konzentration von Lot 1213063 bestätigen zu können:

    1.283 mg/mL.

    Ich hoffe, dies wird Ihnen beim Einstellen der Isotypkontrolle weiterhelfen. Bitte beachte...

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    Thank you for contacting us.

    I advise not buying ab52920 if you want to stain all CD18. The antibody is raised against a phosphorylated site on CD18, and will only stain CD18 that is phosphorylated at that site, not total CD18.

    Th...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has...

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    Thank you for contacting Abcam.

    For ab63388 you get 100 µg at 1mg/ml. For ab52920 you get 100ul butthe concentration varies slightly from batch to batch and so if you could let me know the lot number that you currently have, I will be h...

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    Thank you for contacting us. ab63388 hasn't been tested in WB so we can’t confirm the suitability of this antibody due to lack of tested data. However this does not mean this cannot be used in WB this only means we have not tested it. If you are ...

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    The ab52920 can be used in Western Blot, Immunohistochemistry (paraffin embedded tissue sections, immunoprecipitation and Immunocytochemistry / Immunofluorescence. I hope this will help!

    Thank you for contacting us. Could you let us know the phosphorylated amino acid you are interested in? The integrin beta 2 shows phosphorylation at Serine 745, 756 and at Threonine 758, 759 and 760. The antibodies we have are ab52920 and ab63388 I hop...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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