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Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.
Our Abpromise guarantee covers the use of ab31947 in the following tested applications.
|Flow Cyt||Use 10µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/2000 - 1/4000. Detects a band of approximately 20 kDa (predicted molecular weight: 61 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/400.|
ab31947 staining CD19 in the peripheral blood mononuclear cells from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with paraformaldehyde ,permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% serum for 20 minutes at 25°C.Samples were incubated with primary antibody (1/250 in PBS + 1% goat serum) for 16 hours at 4 °C.A Cy3®-conjugated Goat anti mouse polyclonal was used as the secondary antibody.