Overview

  • Product name
    Anti-CD19 antibody [2E2B6B10]
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [2E2B6B10] to CD19
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, WB, IHC-P, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Human, Recombinant fragment
  • Immunogen

    Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.

  • Positive control
    • Recombinant human CD19 and normal human lymph node tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab31947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB 1/2000 - 1/4000. Detects a band of approximately 20 kDa (predicted molecular weight: 61 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200 - 1/400.
ELISA 1/10000.

Target

  • Function
    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in disease
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [2E2B6B10] images

  • Overlay histogram showing peripheral blood lymphocytes stained with ab31947 (red line). The cells were incubated with the antibody (ab31947, 10µl/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

  • Immnohistochemical staining of paraffin embedded normal human, adult lymph node tissue, using ab31947. Showing cytoplasmic staining. Secondary staining was carried out using an HRP conjugated antibody before colour development using DAB.
  • ab31947 staining CD19 in the peripheral blood mononuclear cells from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with paraformaldehyde ,permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% serum for 20 minutes at 25°C.Samples were incubated with primary antibody (1/250 in PBS + 1% goat serum) for 16 hours at 4 °C.A Cy3®-conjugated Goat anti mouse polyclonal was used as the secondary antibody.

References for Anti-CD19 antibody [2E2B6B10] (ab31947)

This product has been referenced in:
  • Bourgine PE  et al. Osteoinductivity of engineered cartilaginous templates devitalized by inducible apoptosis. Proc Natl Acad Sci U S A 111:17426-31 (2014). IHC-P ; Human . Read more (PubMed: 25422415) »
  • Sohal SS  et al. Evaluation of epithelial mesenchymal transition in patients with chronic obstructive pulmonary disease. Respir Res 12:130 (2011). Read more (PubMed: 21970519) »

See all 5 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Peripheral blood mononuclear cells (PBMCs))
Permeabilization
Yes - 0.5% TX100
Specification
Peripheral blood mononuclear cells (PBMCs)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 04 2016

Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Human blood film)
Specification
Human blood film
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Specification
Human peripheral blood mononuclear cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood lymphocytes)
Specification
Peripheral blood lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll/Hypaque density centrifugation. RBC lysis.
Sample buffer: PBS
Permeabilization
No
Gating Strategy
FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
Username

Dr. Josh Gregorio

Verified customer

Submitted Aug 21 2012

Vielen Dank für Ihre Anfrage.

Leider haben wir nicht die unterschiedlichen Phänotypen dieser Proteine getestet. Da es sich allerdings ja um polyklonale Antikörper gegen das Vollprotein handelt, sollten die meisten Varianten erkannt werden, s...

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Thank you for your enquiry. I can confirm that the storage buffer is made of PBS containing 0.03% azide, therefore we do not recommend its direct use in cell cultures as it is cytotoxic; I would recommend to dialyse the antibody to remove the azide...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Chronic lymphocytic leukaemia cells)
Loading amount
18 µg
Specification
Chronic lymphocytic leukaemia cells
Gel Running Conditions
Reduced Denaturing (12% Tris-Glycine, 60 minutes, 200 volts constant)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 13 2008

Thank you for your enquiry. We have been informed that ab31947 is stored in PBS (without BSA). Please don't hesitate to contact us again if you have further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat brain sections)
Specification
Rat brain sections
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Background Sniper- Biocare Medical REF BS966L as blocking agent for 10 minute(s) · Concentration: 000% · Temperature: 25°C
Username

Mr. osama mohsen

Verified customer

Submitted Oct 02 2007

We have many products in catalogue. Please find below few of these

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http://www.abcam.com/cd19-antibody-epr5906-ab134114.html

http://www.abcam.com/cd19-antibody-2e2b6b10-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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