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Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.
Our Abpromise guarantee covers the use of ab31947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/2000 - 1/4000. Detects a band of approximately 20 kDa (predicted molecular weight: 61 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/200 - 1/400.|
ab31947 staining CD19 in the peripheral blood mononuclear cells from Human by ICC/IF (Immunocytochemistry/immunofluorescence).Cells were fixed with paraformaldehyde ,permeabilized with 0.5% Triton X-100 in PBS and blocked with 5% serum for 20 minutes at 25°C.Samples were incubated with primary antibody (1/250 in PBS + 1% goat serum) for 16 hours at 4 °C.A Cy3®-conjugated Goat anti mouse polyclonal was used as the secondary antibody.