Overview

  • Product nameAnti-CD19 antibody [6D5]
    See all CD19 primary antibodies
  • Description
    Rat monoclonal [6D5] to CD19
  • SpecificityCD19 B-cell differentiation antigen
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CD19-expressing K562 human erythroleukemia cells

Properties

Applications

Our Abpromise guarantee covers the use of ab25232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [6D5] images

  • Immunohistochemical analysis of renal lymph node with PC3-tumour metastases, staining CD19 with ab25232.

    Cryosections were stored at -80°C and incubated with primary antibodies for 1 hour. After washing with PBS, sections were stained for 1 hour with secondary antibodies.
  • Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD19 with ab25232 at 1 μg/106 cells dilution, followed by Rat Anti-Mouse CD3ε-FITC then by Mouse Anti-Rat IgG2a-PE.

References for Anti-CD19 antibody [6D5] (ab25232)

This product has been referenced in:
  • Angelini DJ  et al. Hypoxia-induced mitogenic factor (HIMF/FIZZ1/RELMa) in chronic hypoxia- and antigen-mediated pulmonary vascular remodeling. Respir Res 14:1 (2013). ICC/IF ; Mouse . Read more (PubMed: 23289668) »
  • Donat U  et al. Preferential colonization of metastases by oncolytic vaccinia virus strain GLV-1h68 in a human PC-3 prostate cancer model in nude mice. PLoS One 7:e45942 (2012). Read more (PubMed: 23049897) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer
Permeabilization No
Specification Lung
Blocking step Serum as blocking agent for 1 hour(s) and 5 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 17 2015

We have one unconjugated antibody ab25232 which unfortunately hasn't been tested in functional assays though it has similar clone number then the tested conjugated ones.

In case you are interested in buying and trying this antibody then I wou...

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Thank you for contacting us.

Please check the following antibodies;


http://www.abcam.com/cd19-antibody-6d5-pe-cy5-5-ab25548.html

http://www.abcam.com/cd19-antibody-mb19-1-pe-cy5-5-ab25384.html

I hope this inform...

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Thank you very much for your interest in ab25232.
To our knowledge, ab25232 has not been tested in IHC-P yet.
We do offer the following antibody that is tested and guaranteed for mouse and IHC-P and might be suitable for your purpose of ident...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"