Anti-CD19 antibody [A3-B1] (Allophycocyanin) (ab91178)

Overview

  • Product name
    Anti-CD19 antibody [A3-B1] (Allophycocyanin)
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [A3-B1] to CD19 (Allophycocyanin)
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This information is considered to be commercially sensitive

  • Positive control
    • Normal Human peripheral blood

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 0.9% BSA, 0.15M Sodium chloride, 0.02M Sodium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab91178 is B lineage specific and reacts with early B cell precursors, pre pre B cells, pre B cells, B cells, intermediate B cells, mature B cells and some plasmacytoid cells. Plasma cells were found to be negative. ab91178 does not react with other haemopoïetic cells. ab91178 also reacts with pre B cell lines, B lymphoblastoid cell lines and Burkitt cell lines, and with 50% of myeloma cell lines. Virtually all non T ALL, B CLL and B cell lymphomas were found to be positive, myeloma cells were found to be negative.
  • Clonality
    Monoclonal
  • Clone number
    A3-B1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20 µl for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
    • Involvement in disease
      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antibody deficiency due to defect in CD19, included antibody
      • AW495831 antibody
      • B lymphocyte antigen CD19 antibody
      • B lymphocyte surface antigen B4 antibody
      • B-lymphocyte antigen CD19 antibody
      • B-lymphocyte surface antigen B4 antibody
      • B4 antibody
      • CD19 antibody
      • CD19 antigen antibody
      • CD19 molecule antibody
      • Cd19 protein antibody
      • CD19_HUMAN antibody
      • CVID3 antibody
      • Differentiation antigen CD19 antibody
      • Leu 12 antibody
      • Leu-12 antibody
      • Leu12 antibody
      • MGC109570 antibody
      • MGC12802 antibody
      • T-cell surface antigen Leu-12 antibody
      see all

    Images

    • ab91178, at 20 µl/106, staining CD19 in normal Human peripheral blood by Flow cytometry.

    References

    ab91178 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. I have checked with the originator of the product and it seems that we have an error on our datasheet. This antibody was made from whole cells not with a peptide sequence. Also, epitope mapping was not determined. Theref...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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