Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)

Overview

  • Product nameAnti-CD19 antibody [A3-B1] (CF405M)
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [A3-B1] to CD19 (CF405M)
  • ConjugationCF405M. Ex: 408nm, Em: 452nm
  • SpecificityDirected against the CD19, which is expressed on human B lymphocytes. ab117733 is B lineage-specific and reacts with early B-cell precursors, pre-pre-B-cells, pre-B- cells, B-cells, intermediate B-cells, mature B-cells and some plasmacytoid cells. Plasma cells were found to be negative. It does not react with other haemopoïetic cells. Also reacts with pre-B-cell- lines, B lymphoblastoid cell-lines and Burkitt cell- lines, and with 50% of myeloma cell-lines. Virtually all non T-ALL, B-CLL and B-cell lymphomas were found to be positive, myeloma cells were found to be negative.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CD19 antigen which is expressed on human B lymphocytes.

  • Positive control
    • Normal Human peripheral blood
  • General notes

    CD19 cluster expressed from the earliest stages of B-progenitor development and on all peripheral B cells including germinal centre B cells, all B cell lines tested and B cell leukaemias tested. The antigen is lost on B cell maturation to plasma cells.

    Fluorochrome: CF405M (Abs/Em Max: 408/450 nm) CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450

Properties

Applications

Our Abpromise guarantee covers the use of ab117733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab126036-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [A3-B1] (CF405M) images

  • Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).
  • Staining of CD19 using Normal Human peripheral blood with ab117733 (5 µl/106 cells).

References for Anti-CD19 antibody [A3-B1] (CF405M) (ab117733)

ab117733 has not yet been referenced specifically in any publications.

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