Overview

  • Product nameAnti-CD19 antibody
    See all CD19 primary antibodies
  • Description
    Rabbit polyclonal to CD19
  • Tested applicationsSuitable for: IP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of human CD19.

  • Positive control
    • Jurkat cell line lysates and 293 cell lysates transiently transfected with the CD19 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab76591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 61 kDa.
ELISA 1/1000.

Target

  • FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody images

  • CD19 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to CD19 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76591.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 61kDa; CD19

  • Anti-CD19 antibody (ab76591) at 1/60 dilution + Jurkat cell line lysates at 35 µg

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-CD19 antibody (ab76591) at 1/50 dilution

    Lane 1 : Non transfected 293 cell lysates
    Lane 2 : CD19 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

References for Anti-CD19 antibody (ab76591)

ab76591 has not yet been referenced specifically in any publications.

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