Anti-CD19 antibody [LT19] (Allophycocyanin) (ab18224)

Overview

  • Product nameAnti-CD19 antibody [LT19] (Allophycocyanin)
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [LT19] to CD19 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    B lymphoblast cell line Daudi.

  • Positive control
    • Human blood cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
  • Application notesFlow Cyt: Use 20µl for 100µl human blood cells.
    IP: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
    • Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antibody deficiency due to defect in CD19, included antibody
      • AW495831 antibody
      • B lymphocyte antigen CD19 antibody
      • B lymphocyte surface antigen B4 antibody
      • B-lymphocyte antigen CD19 antibody
      • B-lymphocyte surface antigen B4 antibody
      • B4 antibody
      • CD19 antibody
      • CD19 antigen antibody
      • CD19 molecule antibody
      • Cd19 protein antibody
      • CD19_HUMAN antibody
      • CVID3 antibody
      • Differentiation antigen CD19 antibody
      • Leu 12 antibody
      • Leu-12 antibody
      • Leu12 antibody
      • MGC109570 antibody
      • MGC12802 antibody
      • T-cell surface antigen Leu-12 antibody
      see all

    Anti-CD19 antibody [LT19] (Allophycocyanin) images

    • Flow cytometry analysis of CLL024 cells loaded with control phage, staining CD19 with ab18224.

    References for Anti-CD19 antibody [LT19] (Allophycocyanin) (ab18224)

    This product has been referenced in:
    • Liu L  et al. Pfit is a structurally novel Crohn's disease-associated superantigen. PLoS Pathog 9:e1003837 (2013). Read more (PubMed: 24385909) »
    • Trepel M  et al. Phenotypic detection of clonotypic B cells in multiple myeloma by specific immunoglobulin ligands reveals their rarity in multiple myeloma. PLoS One 7:e31998 (2012). Flow Cyt ; Human . Read more (PubMed: 22384124) »

    See all 2 Publications for this product

    Product Wall



    Ab18224 is a suitable alternative to target CD19 with Flow Cytometry. I’ve conducted a search for an isotype control and found the following product that matches the requirements needed: http://www.abcam.com/mouse-igg1-1f8-allophycocyan...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"