Anti-CD19 antibody [MB19-1] (FITC) (ab24936)

Overview

  • Product nameAnti-CD19 antibody [MB19-1] (FITC)
    See all CD19 primary antibodies
  • Description
    Mouse monoclonal [MB19-1] to CD19 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Dog
    Predicted to work with: Human, Cynomolgus Monkey
  • Immunogen

    Tissue/ cell preparation: CD19+ mouse pre B cell line, 300.191.

Properties

Applications

Our Abpromise guarantee covers the use of ab24936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab37347 - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.

Target

  • FunctionAssembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • Involvement in diseaseDefects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

Anti-CD19 antibody [MB19-1] (FITC) images

  • ab24936 used in Flow Cytometry. Red: ab24936 at a 1/100 dilution staining CD19 in murine lymph cells. Cells were mechanically dissociated from the lymph node and analysed live. Black: Isotype control.

    See Abreview

  • ab24936 used in Flow Cytometry.
    Canine blood leucocytes were harvested in PBS, 2% BSA, 0.01% sodium azide after red blood cell lysis, then fixed with paraformaldehyde. ab24936 used at a 1/100 dilution for 1 hour at 4°C.

    The gating strategy used was for lymphocytes.

    See Abreview

  • Flow Cytometry analysis of BALB/c mouse splenocytes cells labeling CD19 with ab24936 at 1 μg/106 cells dilution. A Rat Anti-Mouse CD3ε-PE was used as the secondary antibody.

References for Anti-CD19 antibody [MB19-1] (FITC) (ab24936)

This product has been referenced in:
  • Belharazem D  et al. cFLIP overexpression in T cells in thymoma-associated myasthenia gravis. Ann Clin Transl Neurol 2:894-905 (2015). Flow Cyt ; Human . Read more (PubMed: 26401511) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Dec 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Rabbit Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Dec 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes and neutrophils
Username

Abcam user community

Verified customer

Submitted Nov 02 2012

Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 13 2012

My recommendations are as follows:

Anti-Mouse IgG1 secondary, pre-adsorbed, FITC-conjugate - ab98692
Anti-Mouse IgG1 secondary, pre-adsorbed, APC-conjugate - We unfortunately do not have an APC conjugate.
Mouse IgG1 isotype control...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: cells from buffy coat
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Nov 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (blood leucocytes)
Specification blood leucocytes
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 2% sodium azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy lymphocytes
Username

Abcam user community

Verified customer

Submitted Aug 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cells from lymph node)
Specification cells from lymph node
Preparation Cell harvesting/tissue preparation method: mechanical dissociation of the lymph node
Sample buffer: PBS/BSA 0.1%
Permeabilization No
Gating Strategy on alive (PI-) cells
Username

Abcam user community

Verified customer

Submitted Jun 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"