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Tissue/ cell preparation: CD19+ mouse pre B cell line, 300.191.
Our Abpromise guarantee covers the use of ab24936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab37347 - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.
ab24936 used in Flow Cytometry. Red: ab24936 at a 1/100 dilution staining CD19 in murine lymph cells. Cells were mechanically dissociated from the lymph node and analysed live. Black: Isotype control.
Flow Cytometry analysis of BALB/c mouse splenocytes cells labeling CD19 with ab24936 at 1 μg/106 cells dilution. A Rat Anti-Mouse CD3ε-PE was used as the secondary antibody.