The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Involvement in disease
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Flow cytometric analysis of normal Human peripheral blood cells with Anti-Human CD3 FITC and mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (left) or ab155357 (right). Cells in the lymphocyte gate were used for analysis.