Overview

  • Product nameAnti-CD1a antibody [CD1a007]
    See all CD1a primary antibodies
  • Description
    Mouse monoclonal [CD1a007] to CD1a
  • SpecificityCD1a staining has been shown to be useful in the differentiation of Langerhans cells from interdigitating cells. It has also proved useful for phenotyping Langerhans cell histiocytosis.
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Prokaryotic recombinant fusion protein corresponding to the external portion of the CD1a molecule

  • Positive control
    • Skin or tonsil

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.30
    Preservative: 0.09% Sodium azide
    Constituent: Tissue culture supernatant
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberCD1a007
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT.
IHC-Fr 1/25 - 1/50. For an ABC system, incubate for 30-60 minutes at RT.

Target

  • FunctionAntigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificityExpressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
  • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localizationCell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 1a antibody
    • CD1 antibody
    • CD1a antibody
    • CD1A Antigen antibody
    • CD1A antigen, a polypeptide antibody
    • CD1a molecule antibody
    • CD1A_HUMAN antibody
    • cluster of differentiation 1 A antibody
    • cortical thymocyte antigen CD1A antibody
    • differentiation antigen CD1 alpha 3 antibody
    • epidermal dendritic cell marker CD1a antibody
    • FCB 6 antibody
    • FCB6 antibody
    • HTA 1 antibody
    • HTA1 antibody
    • hTa1 thymocyte antigen antibody
    • OTTHUMP00000018907 antibody
    • R 4 antibody
    • R4 antibody
    • T 6 antibody
    • T-cell surface antigen T6/Leu-6 antibody
    • T-cell surface glycoprotein CD1a antibody
    • T6 antibody
    see all

Anti-CD1a antibody [CD1a007] images

  • Ab708 positively staining acetone fixed, frozen human skin sections (1/25).

    The secondary Ab used was biotin conjugated anti mouse (1/1) and formed part of of a commercially available kit (Vector labs RTU ABC kit).

    This image is courtesy of an Abreview submitted on 11 October 2005. We do not have any further information relating to this image.

References for Anti-CD1a antibody [CD1a007] (ab708)

This product has been referenced in:
  • Mazal PR  et al. Langerhans cell histiocytosis of the hypothalamus: diagnostic value of immunohistochemistry. Clin Neuropathol 15:87-91 (1996). Read more (PubMed: 8925602) »
  • Fivenson DP & Nickoloff BJ Distinctive dendritic cell subsets expressing factor XIIIa, CD1a, CD1b and CD1c in mycosis fungoides and psoriasis. J Cutan Pathol 22:223-8 (1995). Read more (PubMed: 7593815) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5%
Username

Dr. Julie Richards

Verified customer

Submitted Mar 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1 %
Username

Abcam user community

Verified customer

Submitted Oct 11 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"