Anti-CD1a antibody [EP3091] (ab76531)
Key features and details
- Rabbit monoclonal [EP3091] to CD1a
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CD1a antibody [EP3091]
See all CD1a primary antibodies -
Description
Rabbit monoclonal [EP3091] to CD1a -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT4, K562, fetal thymus lysates, CD1A knockout Jurkat cell lysate and HeLa cell lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP3091 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
Target
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Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 909 Human
- Omim: 188370 Human
- SwissProt: P06126 Human
- Unigene: 1309 Human
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Alternative names
- CD 1a antibody
- CD1 antibody
- CD1a antibody
see all
Images
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All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/1000 dilution
Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : CD1A knockout Jurkat cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L preabsorbed (ab216773) and Goat anti-Mouse IgG H&L preabsorbed (ab216776) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD1a antibody [EP3091] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76531 was shown to bind specifically to CD1a.
A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.
Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-CD1a antibody [EP3091] (ab76531) at 1/5000 dilution
Lane 1 : MOLT4 lysate
Lane 2 : K562 lysate
Lane 3 : Fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab76531 has been referenced in 2 publications.
- Gatti E Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases. Methods Mol Biol 1988:259-270 (2019). PubMed: 31147945
- Mahiddine K et al. CD99 isoforms regulate CD1a expression in human monocyte-derived DCs through ATF-2/CREB-1 phosphorylation. Eur J Immunol 46:1460-71 (2016). PubMed: 27094031