Overview

  • Product nameAnti-CD1a antibody [EP3622]
    See all CD1a primary antibodies
  • Description
    Rabbit monoclonal [EP3622] to CD1a
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in human CD1a

  • Positive control
    • Human fetal thymus lysate, human T-78 cell lysate, human skin tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 1.30 x 10 -11 M Learn more about KD
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP3622
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionAntigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
    • Tissue specificityExpressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localizationCell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. Localizes to cell surface lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 1a antibody
      • CD1 antibody
      • CD1a antibody
      • CD1A Antigen antibody
      • CD1A antigen, a polypeptide antibody
      • CD1a molecule antibody
      • CD1A_HUMAN antibody
      • cluster of differentiation 1 A antibody
      • cortical thymocyte antigen CD1A antibody
      • differentiation antigen CD1 alpha 3 antibody
      • epidermal dendritic cell marker CD1a antibody
      • FCB 6 antibody
      • FCB6 antibody
      • HTA 1 antibody
      • HTA1 antibody
      • hTa1 thymocyte antigen antibody
      • OTTHUMP00000018907 antibody
      • R 4 antibody
      • R4 antibody
      • T 6 antibody
      • T-cell surface antigen T6/Leu-6 antibody
      • T-cell surface glycoprotein CD1a antibody
      • T6 antibody
      see all

    Anti-CD1a antibody [EP3622] images

    • ab108309, at 1/250, staining CD1a in human skin tissue by immunohistochemistry
    • All lanes : Anti-CD1a antibody [EP3622] (ab108309) at 1/1000 dilution

      Lane 1 : Human fetal thymus lysate
      Lane 2 : T-78 cell lysate at 10 µg


      Performed under reducing conditions.

      Predicted band size : 37 kDa
    • ab108309 showing negative staining in Normal stomach tissue.

    • ab108309 showing negative staining in Normal uterus tissue.

    • ab108309 showing positive staining in Normal tonsil tissue.

    • ab108309 showing negative staining in Normal breast tissue.

    • ab108309 showing negative staining in Normal kidney tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD1a antibody [EP3622] (ab108309)

    This product has been referenced in:
    • Nguyen N  et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 38:1074-84 (2016). Human . Read more (PubMed: 26879675) »
    • Tam J  et al. Reconstitution of full-thickness skin by microcolumn grafting. J Tissue Eng Regen Med N/A:N/A (2016). IHC-P ; Human . Read more (PubMed: 27296503) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"