The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells or 100µl of whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCD1B and CD1C are members of the CD1 family of major histocompatibility (MHC)-like glycoproteins. CD1 proteins mediate the presentation of lipid and glycolipid antigens to T cells. The CD1B protein localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens. The human genome contains five CD1 family genes, clustered on chromosome 1. CD1 family members are thought to differ in their cellular localization and specificity for lipid ligands.
Cellular localizationSingle-pass type I membrane protein.
A typical staining pattern with the B-B5 monoclonal antibody of Molt 4 cell lineAb27296, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD1b+c by Flow Cytometry.
References for Anti-CD1b+c antibody [B-B5] (ab27296)
This product has been referenced in:
Groot F et al. Lactoferrin prevents dendritic cell-mediated human immunodeficiency virus type 1 transmission by blocking the DC-SIGN--gp120 interaction. J Virol79:3009-15 (2005).
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