The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells.
Use 10µl for 106 cells, or 100µl of whole blood.
ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionCD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
FACS analysis of CD2 expression in human peripheral blood lymphocytes using biotin conjugated ab27994 at 10µl for 106 cells.
References for Anti-CD2 antibody [LT2] (Biotin) (ab27994)
This product has been referenced in:
Lin CW et al. CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood106:3567-74 (2005).
Read more (PubMed: 16046525) »
Beyers AD et al. Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen. Immunol Rev111:59-77 (1989).
Read more (PubMed: 2576418) »