The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Flow cytometric analysis of normal Human peripheral blood cells with mouse IgG1 kappa Isotype Control APC (open histogram) or ab155364 (filled histogram). Cells in the lymphocyte gate were used for analysis.