The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 20µl for 106 cells. ab118658-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.