Anti-CD20 antibody (ab27093)
Key features and details
- Rabbit polyclonal to CD20
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD20 antibody
See all CD20 primary antibodies -
Description
Rabbit polyclonal to CD20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD20 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P11836 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab27093 is an affinity purified IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
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IHC-P |
1/300. Antigen retrieval is not essential but may optimise staining.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa. |
IHC-P
1/300. Antigen retrieval is not essential but may optimise staining. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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Formalin fixed, paraffin embedded human tonsil tissue slide stained with anti CD20 antibody (ab27093).
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Anti-CD20 antibody (ab27093) at 1/25 dilution + Raji cell lysate
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab27093 has been referenced in 9 publications.
- Sirivisoot S et al. Evaluation of 41 single nucleotide polymorphisms in canine diffuse large B-cell lymphomas using MassARRAY. Sci Rep 12:5120 (2022). PubMed: 35332215
- Wei ZW et al. Immune-infiltration based signature as a novel prognostic biomarker in gastrointestinal stromal tumour. EBioMedicine 57:102850 (2020). PubMed: 32574962
- Webb GM et al. The human IL-15 superagonist ALT-803 directs SIV-specific CD8+T cells into B-cell follicles. Blood Adv 2:76-84 (2018). IHC-P ; Rhesus monkey . PubMed: 29365313
- Stahl EC et al. Evaluation of the host immune response to decellularized lung scaffolds derived from a-Gal knockout pigs in a non-human primate model. Biomaterials 187:93-104 (2018). PubMed: 30312852
- Miles B et al. Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection. PLoS Pathog 12:e1005924 (2016). IHC (PFA fixed) ; Rhesus monkey . PubMed: 27716848
- Miles B et al. Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection. Nat Commun 6:8608 (2015). PubMed: 26482032
- Bojarczuk K et al. B-cell receptor pathway inhibitors affect CD20 levels and impair antitumor activity of anti-CD20 monoclonal antibodies. Leukemia N/A:N/A (2014). Human . PubMed: 24492323
- Connick E et al. Compartmentalization of simian immunodeficiency virus replication within secondary lymphoid tissues of rhesus macaques is linked to disease stage and inversely related to localization of virus-specific CTL. J Immunol 193:5613-25 (2014). PubMed: 25362178
- Moll NM et al. Imaging correlates of leukocyte accumulation and CXCR4/CXCL12 in multiple sclerosis. Arch Neurol 66:44-53 (2009). IHC-Fr ; Human . PubMed: 19139298