The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 19139298
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
Use at an assay dependent concentration.
1/300. Antigen retrieval is not essential but may optimise staining.
FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
Tissue specificityExpressed on B-cells.
Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similaritiesBelongs to the MS4A family.
Post-translational modificationsPhosphorylated. Might be functionally regulated by protein kinase(s).
Bojarczuk K et al. B-cell receptor pathway inhibitors affect CD20 levels and impair antitumor activity of anti-CD20 monoclonal antibodies. LeukemiaN/A:N/A (2014).
Read more (PubMed: 24492323) »
Connick E et al. Compartmentalization of simian immunodeficiency virus replication within secondary lymphoid tissues of rhesus macaques is linked to disease stage and inversely related to localization of virus-specific CTL. J Immunol193:5613-25 (2014).
Read more (PubMed: 25362178) »