Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (ab171203)
Key features and details
- Rat monoclonal [AISB12] to CD20 - Low endotoxin, Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free
See all CD20 primary antibodies -
Description
Rat monoclonal [AISB12] to CD20 - Low endotoxin, Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- A20 cell lysate; BALB/c splenocytes.
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General notes
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS
Aqueous buffer, no sodium azide. -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
AISB12 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
Assay dependent |
|
Flow Cyt |
Use 0.25µg for 105-8 cells.
(in a final volume of 100 μL). ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa. Assay dependent |
Flow Cyt
Use 0.25µg for 105-8 cells. (in a final volume of 100 μL). ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12482 Mouse
- SwissProt: P19437 Mouse
- Unigene: 4046 Mouse
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Images
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All lanes : Anti-CD20 antibody [AISB12] - Low endotoxin, Azide free (ab171203)
Lane 1 : Balb/c thymus cell lysate
Lane 2 : A20 cell lysate
Predicted band size: 32 kDa -
Flow cytometric analysis of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor® 647 and 1.0 ug ab171203 followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.
Datasheets and documents
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Datasheet download
References (1)
ab171203 has been referenced in 1 publication.
- Wang X et al. Technetium-99m-labeled rituximab for use as a specific tracer of sentinel lymph node biopsy: a translational research study. Oncotarget 7:38810-38821 (2016). PubMed: 27246977