The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
Use 0.25µg for 105-8 cells.
(in a final volume of 100 μL).
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
This protein may be involved in the regulation of B-cell activation and proliferation.
Expressed on B-cells.
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Belongs to the MS4A family.
Phosphorylated. Might be functionally regulated by protein kinase(s).
Flow cytometric analysis of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor® 647 and 1.0 ug ab171203 followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.