Anti-CD20 antibody [AT80] (FITC) (ab27856)

Overview

  • Product nameAnti-CD20 antibody [AT80] (FITC)
    See all CD20 primary antibodies
  • Description
    Mouse monoclonal [AT80] to CD20 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityRecognizes the human CD20 cell surface antigen, a glycoprotein of 35-37kD. Although it has not been epitope mapped, we believe that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    NS-O cells transfected with human CD20.

  • Positive control
    • Human peripheral blood cells
  • General notes

    Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).

Properties

Applications

Our Abpromise guarantee covers the use of ab27856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl of neat solution or dilute to a maximum of 1/10 to label 106 cells or 100µl whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
    • Tissue specificityExpressed on B-cells.
    • Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similaritiesBelongs to the MS4A family.
    • Post-translational
      modifications
      Phosphorylated. Might be functionally regulated by protein kinase(s).
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    Anti-CD20 antibody [AT80] (FITC) images

    References for Anti-CD20 antibody [AT80] (FITC) (ab27856)

    ab27856 has not yet been referenced specifically in any publications.

    Product Wall

    I am sorry about that mix up, I mistyped the abnumber. Thank you for bringing it to my attention.   Again, this type of assay has not been tested by our lab but I have spoken with the lab and can offer you the following advice. That being said, I wo...

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    Thank you for your inquiry about ab27856. I've spoken with the lab about this and received the following response: This product has not been tested for neutralization assays. We have only tested it for use with flow cytometry. I’m guessing you are in...

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    Thank you for your enquiry. Although ab27856 has not been epitope mapped, the originator believes that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining. For ab1169, th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"