Anti-CD20 antibody [B9E9] (FITC) (ab1169)

Overview

  • Product nameAnti-CD20 antibody [B9E9] (FITC)
    See all CD20 primary antibodies
  • Description
    Mouse monoclonal [B9E9] to CD20 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityIt is strictly specific to an extracellular epitope of human CD20 antigen, present on normal peripheral B lymphocytes, malignant cells of pre-B acute lymphoblastic leukaemia patients and malignant cells of B-type chronic lymphoblastic leukaemia patients. It is not necessary to permeabilize the cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Cells of lymphoblastoid cell line DAUDI

Properties

Applications

Our Abpromise guarantee covers the use of ab1169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-5µl for 106 cells. Optimal volumes should be determined by the end user.

Target

  • FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificityExpressed on B-cells.
  • Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesBelongs to the MS4A family.
  • Post-translational
    modifications
    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Anti-CD20 antibody [B9E9] (FITC) images

  • Overlay histogram showing peripheral blood lymphocytes stained with ab1169 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1169, 0.06 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a FITC (1 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

References for Anti-CD20 antibody [B9E9] (FITC) (ab1169)

ab1169 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Although ab27856 has not been epitope mapped, the originator believes that it recognizes a region on the extracellular surface of CD20 because no permeabilization treatment is required prior to staining. For ab1169, th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"