The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells in 100µl, or for 100µl of whole blood.
FuncS: Use at an assay dependent dilution.
ab52063 can be used for monitoring of B cell subsets in peripheral blood; analysis of B cell subsets; study of B cell activation; study of B cell neoplasms; study of B cell activation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
Tissue specificityExpressed on B-cells.
Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
Sequence similaritiesBelongs to the MS4A family.
Post-translational modificationsPhosphorylated. Might be functionally regulated by protein kinase(s).