Overview

  • Product name
    Anti-CD20 antibody [EP459Y]
    See all CD20 primary antibodies
  • Description
    Rabbit monoclonal [EP459Y] to CD20
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD20 aa 250 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Raji cell lysate. Human lymph node tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab78237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
IHC-P 1/50 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      This protein may be involved in the regulation of B-cell activation and proliferation.
    • Tissue specificity
      Expressed on B-cells.
    • Involvement in disease
      Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • Sequence similarities
      Belongs to the MS4A family.
    • Post-translational
      modifications
      Phosphorylated. Might be functionally regulated by protein kinase(s).
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MGC3969 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    Anti-CD20 antibody [EP459Y] images

    • Unpurified ab78237 showing positive staining in normal tonsil tissue.

    • Immunohistochemical staining of paraffin embedded B cell lymphoma with purified ab78237 at a working dilution of 1/50. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Anti-CD20 antibody [EP459Y] (ab78237) at 1/10000 dilution (purified) + Raji whole cell lysate

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Unpurified ab78237 staining human CD20 in human lymphoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    • ab78237 (purified) at 1/20 immunoprecipitating CD20 in 10 μg Ramos cell lysate (Lanes 1 and 2, observed at 33 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Anti-CD20 antibody [EP459Y] (ab78237) at 1/5000 dilution (unpurified) + Raji cell lysate

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Unpurified ab78237 showing positive staining in normal spleen tissue.

    • Unpurified ab78237 showing negative staining in normal brain tissue.

    • Unpurified ab78237 showing negative staining in normal heart tissue.

    • Unpurified ab78237 showing negative staining in normal kidney tissue.

    References for Anti-CD20 antibody [EP459Y] (ab78237)

    This product has been referenced in:
    • Su S  et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). IHC ; Human . Read more (PubMed: 28290464) »
    • Dai B  et al. Hepatitis C virus upregulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders. Oncogene 35:2979-90 (2016). IF . Read more (PubMed: 26434584) »

    See all 12 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Marmoset spleen)
    Specification
    Marmoset spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jul 19 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid ph6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jul 19 2010

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Spleen)
    Permeabilization
    Yes - Tween
    Specification
    Spleen
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Username

    Andrea Vecchione

    Verified customer

    Submitted Sep 07 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.2% Triton X-100
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 06 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Sample
    Rhesus monkey Tissue sections (Lymph node)
    Specification
    Lymph node
    Permeabilization
    Yes - 0.5% Triton X
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 19 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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