Overview

  • Product nameAnti-CD20 antibody [SP32]
    See all CD20 primary antibodies
  • Description
    Rabbit monoclonal [SP32] to CD20
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptides from C terminus of human CD20 protein.

  • Positive control
    • Human tonsil and Ramos cell Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/100. Do not perform antigen retrieval.

Target

  • FunctionThis protein may be involved in the regulation of B-cell activation and proliferation.
  • Tissue specificityExpressed on B-cells.
  • Involvement in diseaseDefects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • Sequence similaritiesBelongs to the MS4A family.
  • Post-translational
    modifications
    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • APY antibody
    • ATOPY antibody
    • B lymphocyte antigen CD20 antibody
    • B Lymphocyte Cell Surface Antigen B1 antibody
    • B-lymphocyte antigen CD20 antibody
    • B-lymphocyte cell-surface antigen B1 antibody
    • B-lymphocyte surface antigen B1 antibody
    • B1 antibody
    • Bp 35 antibody
    • Bp35 antibody
    • CD 20 antibody
    • CD20 antibody
    • CD20 antigen antibody
    • CD20 receptor antibody
    • CD20_HUMAN antibody
    • CVID5 antibody
    • Fc epsilon receptor I beta chain antibody
    • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
    • FCER1B antibody
    • High affinity immunoglobulin epsilon receptor subunit beta antibody
    • IgE Fc receptor subunit beta antibody
    • IGEL antibody
    • IGER antibody
    • IGHER antibody
    • LEU 16 antibody
    • LEU16 antibody
    • leukocyte surface antigen Leu 16 antibody
    • Leukocyte surface antigen Leu-16 antibody
    • Ly44 antibody
    • Membrane spanning 4 domains A1 antibody
    • Membrane spanning 4 domains subfamily A member 2 antibody
    • Membrane-spanning 4-domains subfamily A member 1 antibody
    • MS4A1 antibody
    • MS4A2 antibody
    • S7 antibody
    see all

Anti-CD20 antibody [SP32] images

  • Flow cytometric analysis of rabbit anti-CD20 (SP32) antibody ab64088(1/100)  in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of CD20 expression in formalin fixed, paraffin embedded human tonsil tissue using ab64088 at a 1/100 dilution.
  • Anti-CD20 antibody [SP32] (ab64088) at 1/100 dilution + lysate prepared from Ramos cells

    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

References for Anti-CD20 antibody [SP32] (ab64088)

ab64088 has not yet been referenced specifically in any publications.

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