Anti-CD200 / OX2 antibody [MRC OX90] (ab33734)

Overview

  • Product name
    Anti-CD200 / OX2 antibody [MRC OX90]
    See all CD200 / OX2 primary antibodies
  • Description
    Rat monoclonal [MRC OX90] to CD200 / OX2
  • Tested applications
    Suitable for: IHC-FoFr, Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse CD200-rat CD4 fusion protein.

  • General notes


    Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This purified antibody was prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notes
    Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line.
  • Clonality
    Monoclonal
  • Clone number
    MRC OX90
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
  • Sequence similarities
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen identified by monoclonal antibody MRC OX 2 antibody
    • CD200 antibody
    • CD200 antigen antibody
    • CD200 molecule antibody
    • MOX1 antibody
    • MOX2 antibody
    • MRC antibody
    • MRC OX 2 antigen antibody
    • My033 antibody
    • OX 2 antibody
    • OX 2 membrane glycoprotein precursor antibody
    • OX-2 membrane glycoprotein antibody
    • OX2G antibody
    • OX2G_HUMAN antibody
    see all

Anti-CD200 / OX2 antibody [MRC OX90] images

  • Immunohistochemical analysis of murine brain tissue, staining CD200 / OX2 with ab33734.

    Tissue was fixed with paraformaldehyde and blocked with 5% BSA + 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/600 in 5% BSA + 5% NGS) for 17 hours at 4°C. An AlexaFluor®555-conjugated goat anti-rat polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-CD200 / OX2 antibody [MRC OX90] (ab33734)

ab33734 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
BSA + serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Nov 22 2012

DISCOUNT CODE: 40721
Expiration date: 20. November 2012
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