The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Use of an antibody which removes non specific background caused by Fc receptors is recommended.
ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
Tissue specificityExpressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
Sequence similaritiesBelongs to the CD200R family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Post-translational modificationsThe mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.