Anti-CD200R antibody [OX102] (Phycoerythrin) (ab34135)

Overview

  • Product nameAnti-CD200R antibody [OX102] (Phycoerythrin)
    See all CD200R primary antibodies
  • Description
    Mouse monoclonal [OX102] to CD200R (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody is specific for the CD200R ligand.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation: Membrane fraction of thioglycollate elicited rat peripheral cells.

  • General notes


    This antibody has been shown to block the interaction of OX2 ligand with CD200.

Properties

Applications

Our Abpromise guarantee covers the use of ab34135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

Target

  • FunctionReceptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  • Tissue specificityExpressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
  • Sequence similaritiesBelongs to the CD200R family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 200R antibody
    • CD200 cell surface glycoprotein receptor antibody
    • CD200 receptor 1 antibody
    • Cd200r1 antibody
    • Cell surface glycoprotein CD200 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor antibody
    • cell surface glycoprotein receptor CD200 antibody
    • CRTR 2 antibody
    • CRTR2 antibody
    • HCRTR2 antibody
    • MO2R1_HUMAN antibody
    • MOX2 receptor antibody
    • MOX2R antibody
    • OX2R antibody
    see all

Anti-CD200R antibody [OX102] (Phycoerythrin) images

  • Overlay histogram showing Rat blood cells stained with ab34135 (red line). The sample was prepared by lysing Red Blood cells and cells were fixed with formaldehyde. The cells were then incubated with the antibody for 1 hour diluted 1/100 in PBS/1% BSA/0.01% Azide. The cell population was gated on monocytes.

    See Abreview

References for Anti-CD200R antibody [OX102] (Phycoerythrin) (ab34135)

ab34135 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation Formaldehyde
Permeabilization No
Gating Strategy on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (from blood)
Specification from blood
Preparation Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation Formaldehyde
Permeabilization No
Gating Strategy on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Application Flow Cytometry
Sample Mouse Cell (from blood)
Specification from blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation Formaldehyde
Permeabilization No
Gating Strategy on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Permeabilization No
Gating Strategy monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"