Anti-CD200R antibody [OX102] (Phycoerythrin) (ab34135)

Overview

  • Product name
    Anti-CD200R antibody [OX102] (Phycoerythrin)
    See all CD200R primary antibodies
  • Description
    Mouse monoclonal [OX102] to CD200R (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    This antibody is specific for the CD200R ligand.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue/ cell preparation: Membrane fraction of thioglycollate elicited rat peripheral cells.

  • General notes


    This antibody has been shown to block the interaction of OX2 ligand with CD200.

Properties

Applications

Our Abpromise guarantee covers the use of ab34135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.

Target

  • Function
    Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.
  • Tissue specificity
    Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells.
  • Sequence similarities
    Belongs to the CD200R family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 200R antibody
    • CD200 cell surface glycoprotein receptor antibody
    • CD200 receptor 1 antibody
    • Cd200r1 antibody
    • Cell surface glycoprotein CD200 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor 1 antibody
    • Cell surface glycoprotein OX2 receptor antibody
    • cell surface glycoprotein receptor CD200 antibody
    • CRTR 2 antibody
    • CRTR2 antibody
    • HCRTR2 antibody
    • MO2R1_HUMAN antibody
    • MOX2 receptor antibody
    • MOX2R antibody
    • OX2R antibody
    see all

Images

  • Overlay histogram showing Rat blood cells stained with ab34135 (red line). The sample was prepared by lysing Red Blood cells and cells were fixed with formaldehyde. The cells were then incubated with the antibody for 1 hour diluted 1/100 in PBS/1% BSA/0.01% Azide. The cell population was gated on monocytes.

    See Abreview

References

ab34135 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (from blood)
Specification
from blood
Preparation
Cell harvesting/tissue preparation method: after lysis of red blood cells
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Application
Flow Cytometry
Sample
Mouse Cell (from blood)
Specification
from blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
on monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat
Sample buffer: PBS/BSA(1%)/azide(0.01%)
Permeabilization
No
Gating Strategy
monocytes
Username

Abcam user community

Verified customer

Submitted Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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