Anti-CD22 antibody [2D6] (Phycoerythrin) (ab25369)

Overview

  • Product nameAnti-CD22 antibody [2D6] (Phycoerythrin)
    See all CD22 primary antibodies
  • Description
    Rat monoclonal [2D6] to CD22 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityCD22 (150 kDa), a B lymphocyte adhesion molecule
  • Tested applicationsSuitable for: Flow Cyt, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    This antibody has shown to be useful in studies of blocking of B cell adhesion.

Properties

Applications

Our Abpromise guarantee covers the use of ab25369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
Blocking Use at an assay dependent dilution.

Target

  • FunctionMediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • Tissue specificityB-lymphocytes.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Post-translational
    modifications
    Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
    Phosphorylated on tyrosine residues by LYN.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B cell receptor CD22 precursor antibody
    • B lymphocyte cell adhesion molecule antibody
    • B-cell receptor CD22 antibody
    • B-lymphocyte cell adhesion molecule antibody
    • BL CAM antibody
    • BL-CAM antibody
    • BLCAM antibody
    • CD 22 antibody
    • CD22 antibody
    • CD22 antigen antibody
    • CD22 molecule antibody
    • CD22 protein antibody
    • CD22_HUMAN antibody
    • Lectin 2 antibody
    • Leu14 antibody
    • Lyb8 antibody
    • MGC130020 antibody
    • sialic acid binding Ig like lectin 2 antibody
    • Sialic acid binding immunoglobulin like lectin 2 antibody
    • Sialic acid-binding Ig-like lectin 2 antibody
    • SIGLEC 2 antibody
    • Siglec-2 antibody
    • SIGLEC2 antibody
    • T cell surface antigen Leu 14 antibody
    • T-cell surface antigen Leu-14 antibody
    see all

Anti-CD22 antibody [2D6] (Phycoerythrin) images

  • BALB/c splenocytes were double-stained with Rat anti-Mouse CD22 R-PE (ab25369) at 0.3 ug/106 cells and Rat anti-Mouse CD19 FITC. Small lymphocytes were gated and analyzed on a flow cytometer.

References for Anti-CD22 antibody [2D6] (Phycoerythrin) (ab25369)

ab25369 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. This product has been tested on B-6 and BALB/c mice. If you have a certain strain you are looking for, I may be able to find a reference. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"