Overview

  • Product nameAnti-CD22 antibody [EP498Y]
    See all CD22 primary antibodies
  • Description
    Rabbit monoclonal [EP498Y] to CD22
  • Specificityab33859 recognises CD22.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD22 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Raji and Raw264.7 cell lysate and rat spleen tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab33859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionMediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • Tissue specificityB-lymphocytes.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications
      Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell receptor CD22 precursor antibody
      • B lymphocyte cell adhesion molecule antibody
      • B-cell receptor CD22 antibody
      • B-lymphocyte cell adhesion molecule antibody
      • BL CAM antibody
      • BL-CAM antibody
      • BLCAM antibody
      • CD 22 antibody
      • CD22 antibody
      • CD22 antigen antibody
      • CD22 molecule antibody
      • CD22 protein antibody
      • CD22_HUMAN antibody
      • Lectin 2 antibody
      • Leu14 antibody
      • Lyb8 antibody
      • MGC130020 antibody
      • sialic acid binding Ig like lectin 2 antibody
      • Sialic acid binding immunoglobulin like lectin 2 antibody
      • Sialic acid-binding Ig-like lectin 2 antibody
      • SIGLEC 2 antibody
      • Siglec-2 antibody
      • SIGLEC2 antibody
      • T cell surface antigen Leu 14 antibody
      • T-cell surface antigen Leu-14 antibody
      see all

    Anti-CD22 antibody [EP498Y] images

    • All lanes : Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (purified)

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Raw264.7 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 95 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-CD22 antibody [EP498Y] (ab33859) at 1/5000 dilution (purified) + Raji whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 95 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-CD22 antibody [EP498Y] (ab33859) at 1/1000 dilution (unpurified) + Raji cell lysate

      Predicted band size : 95 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    References for Anti-CD22 antibody [EP498Y] (ab33859)

    This product has been referenced in:
    • Bannister D  et al. Epitope mapping and key amino acid identification of anti-CD22 immunotoxin CAT-8015 using hybrid ß-lactamase display. Protein Eng Des Sel 24:351-60 (2011). Read more (PubMed: 21159620) »
    • Cariappa A  et al. B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase. J Exp Med 206:125-38 (2009). Read more (PubMed: 19103880) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab33859.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"