Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)

Overview

  • Product nameAnti-CD22 (phospho Y842) antibody [Y507]
    See all CD22 primary antibodies
  • Description
    Rabbit monoclonal [Y507] to CD22 (phospho Y842)
  • SpecificityThe antibody detects CD22 phosphorylation on Tyrosine 842 and is predicted to detect splice isoforms 2 based on sequence homology.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 842 of human CD22.

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab32355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 95 kDa).

    Is unsuitable for FACS, IHC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
    • Tissue specificityB-lymphocytes.
    • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • DomainContains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
    • Post-translational
      modifications
      Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
      Phosphorylated on tyrosine residues by LYN.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B cell receptor CD22 precursor antibody
      • B lymphocyte cell adhesion molecule antibody
      • B-cell receptor CD22 antibody
      • B-lymphocyte cell adhesion molecule antibody
      • BL CAM antibody
      • BL-CAM antibody
      • BLCAM antibody
      • CD 22 antibody
      • CD22 antibody
      • CD22 antigen antibody
      • CD22 molecule antibody
      • CD22 protein antibody
      • CD22_HUMAN antibody
      • Lectin 2 antibody
      • Leu14 antibody
      • Lyb8 antibody
      • MGC130020 antibody
      • sialic acid binding Ig like lectin 2 antibody
      • Sialic acid binding immunoglobulin like lectin 2 antibody
      • Sialic acid-binding Ig-like lectin 2 antibody
      • SIGLEC 2 antibody
      • Siglec-2 antibody
      • SIGLEC2 antibody
      • T cell surface antigen Leu 14 antibody
      • T-cell surface antigen Leu-14 antibody
      see all

    Anti-CD22 (phospho Y842) antibody [Y507] images

    • All lanes : Anti-CD22 (phospho Y842) antibody [Y507] (ab32355) at 1/2000 dilution

      Lane 1 : Raji Cell Lysate, Untreated
      Lane 2 : Raji Cell Lysate, Treated with Pervanadate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 95 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    References for Anti-CD22 (phospho Y842) antibody [Y507] (ab32355)

    ab32355 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"