Overview

  • Product name
    Anti-CD226 antibody [DX11]
    See all CD226 primary antibodies
  • Description
    Mouse monoclonal [DX11] to CD226
  • Tested applications
    Suitable for: Flow Cyt, Functional Studies, IP, Blockingmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) - cytotoxic T lymphocyte clone.

  • General notes


    This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  • Primary antibody notes
    This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
  • Clonality
    Monoclonal
  • Clone number
    DX11
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Functional Studies Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 23980210

Target

  • Function
    Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificity
    Expressed by peripheral blood T-lymphocytes.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • adhesion glycoprotein antibody
    • CD226 antibody
    • CD226 antigen antibody
    • CD226 molecule antibody
    • CD226_HUMAN antibody
    • DNAM 1 antibody
    • DNAM-1 antibody
    • DNAM1 antibody
    • DNAX accessory molecule 1 antibody
    • platelet and T cell activation antigen 1 antibody
    • PTA1 antibody
    • T lineage specific activation antigen 1 antigen antibody
    • TLiSA1 antibody
    see all

Images

  • Human peripheral blood lymphocytes stained with ab33397, using 10µl of the working dilution for 106 cells in 100µl.

References

This product has been referenced in:
  • Lozano E  et al. The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans. J Immunol 191:3673-80 (2013). Blocking ; Human . Read more (PubMed: 23980210) »
  • Lozano E  et al. The TIGIT/CD226 axis regulates human T cell function. J Immunol 188:3869-75 (2012). Read more (PubMed: 22427644) »

See all 3 Publications for this product

Customer reviews and Q&As

A description of the production and characterization of the CD226 antibody, clone DX11, and the statement regarding inhibition and blocking, are in the following reference.


Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule invol...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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