Anti-CD226 (phospho S329) antibody (ab61790)

Overview

  • Product nameAnti-CD226 (phospho S329) antibody
    See all CD226 primary antibodies
  • Description
    Rabbit polyclonal to CD226 (phospho S329)
  • Specificityab61790 detects CD226 only when phosphorylated at serine 329.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human CD226 around the phosphorylation site of serine 329 (T-F-SP-R-R).

  • Positive control
    • Human brain tissue; extracts from COS7 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • FunctionReceptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
  • Tissue specificityExpressed by peripheral blood T-lymphocytes.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • adhesion glycoprotein antibody
    • CD226 antibody
    • CD226 antigen antibody
    • CD226 molecule antibody
    • CD226_HUMAN antibody
    • DNAM 1 antibody
    • DNAM-1 antibody
    • DNAM1 antibody
    • DNAX accessory molecule 1 antibody
    • platelet and T cell activation antigen 1 antibody
    • PTA1 antibody
    • T lineage specific activation antigen 1 antigen antibody
    • TLiSA1 antibody
    see all

Anti-CD226 (phospho S329) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61790 at 1/50-1/100 dilution with (+) or without (-) immunising phosphopeptide.
  • All lanes : Anti-CD226 (phospho S329) antibody (ab61790) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells
    Lane 2 : Extracts from COS7 cells with the immunising phosphopeptide


    Predicted band size : 39 kDa
    Observed band size : ~ 59 kDa (why is the actual band size different from the predicted?)

References for Anti-CD226 (phospho S329) antibody (ab61790)

ab61790 has not yet been referenced specifically in any publications.

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Merci de votre intérêt pour ab61790.

Cet anticorps n’a pas encore été testé en IHC-Fr et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires.
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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab61790 has never been tested in frozen sections (IHC-Fr). All tested and guaranteed applications are specified on our datasheets, and these are updat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"