The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.1µg for 106 cells.
Note: ab25457 can be used for identification and enumeration of CD23+ cells by flow cytometry and for differentiation of B cell subpopulations by cell sorting.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
Flow cytometry analysis of BALB/c mouse splenocytes labeling CD23 with ab25457 at 0.1 µg/106cells and CD3 epsilon with Rat Anti-Mouse CD3 epsilon-FITC.
References for Anti-CD23 antibody [2G8] (Allophycocyanin) (ab25457)
This product has been referenced in:
Hamel K et al. Suppression of Proteoglycan-Induced Arthritis by Anti-CD20 B Cell Depletion Therapy Is Mediated by Reduction in Autoantibodies and CD4+ T Cell Reactivity. J Immunol180:4994-5003 (2008).
Read more (PubMed: 18354225) »