Recombinant Anti-CD23 antibody [EPR3617] (ab92495)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3617] to CD23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD23 antibody [EPR3617]
See all CD23 primary antibodies -
Description
Rabbit monoclonal [EPR3617] to CD23 -
Host species
Rabbit -
Specificity
Recognizes both membrane bound and soluble forms of CD23. -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD23 aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Human lymph and tonsil lysates; IHC-P: Human tonsil tissue; Flow Cyt: Human peripheral blood mononuclear cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 5.50 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3617 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/00 - 1/250. |
|
Flow Cyt |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
IHC-P
1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/00 - 1/250. |
Flow Cyt
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane. Secreted. Also exists as a soluble excreted form. - Information by UniProt
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Database links
- Entrez Gene: 2208 Human
- Omim: 151445 Human
- SwissProt: P06734 Human
- Unigene: 465778 Human
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Alternative names
- Blast 2 antibody
- BLAST-2 antibody
- Blast2 antibody
see all
Images
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Anti-CD23 antibody [EPR3617] (ab92495) at 1/1000 dilution (Purified) + Human lymph lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 37,45 kDa why is the actual band size different from the predicted?The bands detected by ab92495 are consistent with what have been described in PMID: 20876574 and PMID: 17389606.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD23 with Purified ab92495 at 1/400 dilution (0.5 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Flow Cytometry analysis of Human peripheral blood mononuclear (PBMC) cells labeling CD23 with purified ab92495 at 1:20 dilution (10 µg/ml) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Left).
Cells were stained with rabbit IgG (Left) or ab92495 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.
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ab92495 (unpurified), at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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ab92495 (unpurified) showing positive staining in Normal tonsil tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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ab92495 (unpurified) showing negative staining in Normal heart tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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ab92495 (unpurified) showing negative staining in Normal breast tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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ab92495 (unpurified) showing negative staining in Normal brain tissue.
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab92495 has been referenced in 2 publications.
- Wen S et al. Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney. Front Oncol 10:609839 (2020). PubMed: 33585230
- Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155