Anti-CD23 antibody [EPR3617] (ab92495)

Overview

  • Product nameAnti-CD23 antibody [EPR3617]
    See all CD23 primary antibodies
  • Description
    Rabbit monoclonal [EPR3617] to CD23
  • SpecificityRecognizes both membrane bound and soluble forms of CD23.
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus (extracellular domain) of Human CD23.

  • Positive control
    • WB: Raji, Lymph node and Human tonsil lysates IHC-P: Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
  • Application notesFlow Cyt: 1/20.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/500. Predicted molecular weight: 36 kDa.

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
    • Sequence similaritiesContains 1 C-type lectin domain.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localizationCell membrane. Secreted. Also exists as a soluble excreted form.
    • Information by UniProt
    • Database links
    • Alternative names
      • Blast 2 antibody
      • BLAST-2 antibody
      • Blast2 antibody
      • C type lectin domain family 4 member J antibody
      • C-type lectin domain family 4 member J antibody
      • C-type lectin domain family 4, member J antibody
      • CD 23 antibody
      • CD 23A antibody
      • CD23 antibody
      • CD23 antigen antibody
      • CD23A antibody
      • CLEC 4J antibody
      • CLEC4J antibody
      • Fc epsilon receptor II antibody
      • Fc epsilon RII antibody
      • Fc fragment of IgE low affinity II receptor for antibody
      • Fc fragment of IgE receptor II antibody
      • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
      • Fc of IgE, low affinity II, receptor for (CD23) antibody
      • Fc receptor IgE low affinity II alpha polypeptide antibody
      • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
      • Fc-epsilon-RII antibody
      • FCE 2 antibody
      • FCE2 antibody
      • FCER 2 antibody
      • Fcer2 antibody
      • FCER2_HUMAN antibody
      • FCER2A antibody
      • FceRII antibody
      • IgE binding factor antibody
      • IgE receptor lymphocyte antibody
      • IgE-binding factor antibody
      • IGEBF antibody
      • Immunoglobulin E binding factor antibody
      • Immunoglobulin E receptor, low affinity II antibody
      • Immunoglobulin E-binding factor antibody
      • Immunoglobulin epsilon chain antibody
      • LEUKOCYTE ANTIGEN CD23 antibody
      • Low Affinity IgE Receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
      • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
      • Ly-42 antibody
      • Ly42 antibody
      • Lymphocyte antigen CD23 antibody
      • Lymphocyte IgE receptor antibody
      • MGC93219 antibody
      see all

    Anti-CD23 antibody [EPR3617] images

    • Anti-CD23 antibody [EPR3617] (ab92495) at 1/500 dilution + Human lymph node at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • All lanes : Anti-CD23 antibody [EPR3617] (ab92495) at 1/500 dilution

      Lane 1 : Raji lysate
      Lane 2 : Lymph node lysate
      Lane 3 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • ab92495, at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.
    • ab92495 showing positive staining in Normal tonsil tissue.

    • ab92495 showing negative staining in Normal heart tissue.

    • ab92495 showing negative staining in Normal breast tissue.

    • ab92495 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD23 antibody [EPR3617] (ab92495)

    ab92495 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"