Recombinant
RabMAb

Anti-CD23 antibody [EPR3617] (ab92495)

Overview

  • Product name
    Anti-CD23 antibody [EPR3617]
    See all CD23 primary antibodies
  • Description
    Rabbit monoclonal [EPR3617] to CD23
  • Specificity
    Recognizes both membrane bound and soluble forms of CD23.
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus (extracellular domain) of Human CD23.

  • Positive control
    • WB: Raji, Lymph node and Human tonsil lysates IHC-P: Human tonsil tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
  • Application notes
    Flow Cyt: 1/20.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: 1/500. Predicted molecular weight: 36 kDa.

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
    • Sequence similarities
      Contains 1 C-type lectin domain.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localization
      Cell membrane. Secreted. Also exists as a soluble excreted form.
    • Information by UniProt
    • Database links
    • Alternative names
      • Blast 2 antibody
      • BLAST-2 antibody
      • Blast2 antibody
      • C type lectin domain family 4 member J antibody
      • C-type lectin domain family 4 member J antibody
      • C-type lectin domain family 4, member J antibody
      • CD 23 antibody
      • CD 23A antibody
      • CD23 antibody
      • CD23 antigen antibody
      • CD23A antibody
      • CLEC 4J antibody
      • CLEC4J antibody
      • Fc epsilon receptor II antibody
      • Fc epsilon RII antibody
      • Fc fragment of IgE low affinity II receptor for antibody
      • Fc fragment of IgE receptor II antibody
      • Fc fragment of IgE, low affinity II, receptor for (CD23) antibody
      • Fc of IgE, low affinity II, receptor for (CD23) antibody
      • Fc receptor IgE low affinity II alpha polypeptide antibody
      • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody
      • Fc-epsilon-RII antibody
      • FCE 2 antibody
      • FCE2 antibody
      • FCER 2 antibody
      • Fcer2 antibody
      • FCER2_HUMAN antibody
      • FCER2A antibody
      • FceRII antibody
      • IgE binding factor antibody
      • IgE receptor lymphocyte antibody
      • IgE-binding factor antibody
      • IGEBF antibody
      • Immunoglobulin E binding factor antibody
      • Immunoglobulin E receptor, low affinity II antibody
      • Immunoglobulin E-binding factor antibody
      • Immunoglobulin epsilon chain antibody
      • LEUKOCYTE ANTIGEN CD23 antibody
      • Low Affinity IgE Receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor antibody
      • Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody
      • Low affinity immunoglobulin epsilon Fc receptor soluble form antibody
      • Ly-42 antibody
      • Ly42 antibody
      • Lymphocyte antigen CD23 antibody
      • Lymphocyte IgE receptor antibody
      • MGC93219 antibody
      see all

    Images

    • Anti-CD23 antibody [EPR3617] (ab92495) at 1/500 dilution + Human lymph node at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • All lanes : Anti-CD23 antibody [EPR3617] (ab92495) at 1/500 dilution

      Lane 1 : Raji lysate
      Lane 2 : Lymph node lysate
      Lane 3 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
    • ab92495, at a 1/100 dilution, staining CD23 in formalin fixed, paraffin embedded Human tonsil tissue by Immunohistochemistry. Detection: DAB staining.
    • ab92495 showing positive staining in Normal tonsil tissue.

    • ab92495 showing negative staining in Normal heart tissue.

    • ab92495 showing negative staining in Normal breast tissue.

    • ab92495 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab92495 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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