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|Sample type||Average %||Range|
|Serum||93||81% - 120%|
Abcam’s CD23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD23 concentrations in cell culture supernatant and serum and plasma (EDTA, citrate, heparin).
CD23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD23 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD23 sample captured in plate.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-human CD23 monoclonal antibody||1 x 100µl|
|Blue-Dye||1 x 400µl|
|CD23 Standard Lyophilized (400 U/mL on reconstitution)||2 vials|
|Control high||1 vial|
|Control low||1 vial|
|Green-Dye||1 x 400µl|
|Human anti-CD23 monocloncal antibody coated microplate (12 x 8 wells)||1 unit|
|Red-Dye||1 x 400µl|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119512 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"