Overview

  • Product nameCD23 Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6.3%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    6.8 U/ml
  • Range
    12.5 U/ml - 400 U/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Serum 93 81% - 120%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD23 concentrations in cell culture supernatant and serum and plasma (EDTA, citrate, heparin).

    CD23 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated CD23 detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD23 sample captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionThis receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localizationCell membrane. Secreted. Also exists as a soluble excreted form.
  • Target information above from: UniProt accession P06734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • BLAST 2
    • BLAST-2
    • C-type lectin domain family 4 member J
    • CD23
    • CD23 antigen
    • CD23A
    • CLEC4J
    • Fc fragment of IgE low affinity II receptor for (CD23)
    • Fc-epsilon-RII
    • FCE2
    • Fcer2
    • FCER2_HUMAN
    • IGEBF
    • Immunoglobulin E-binding factor
    • Low affinity immunoglobulin epsilon Fc receptor
    • Low affinity immunoglobulin epsilon Fc receptor soluble form
    • Lymphocyte IgE receptor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

CD23 Human ELISA Kit images

  • Representative Standard Curve using ab119512.

Protocols

References for CD23 Human ELISA Kit (ab119512)

ab119512 has not yet been referenced specifically in any publications.

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