- Datasheet
- Specific References (10)
- Protocols
Overview
-
Product nameAnti-CD24 antibody [SN3] (FITC)
See all CD24 primary antibodies -
DescriptionMouse monoclonal [SN3] to CD24 (FITC)
-
Host speciesMouse
-
ConjugationFITC. Ex: 493nm, Em: 528nm
-
Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Glycoproteins purified from human NALM-1 cell line
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C.
-
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
-
ClonalityMonoclonal
-
Clone numberSN3
-
IsotypeIgG1
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab30350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. 1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | Use at an assay dependent concentration. Based on customer feedback, the use of a secondary antibody may be necessary |
Target
-
FunctionModulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
-
Tissue specificityB-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
-
Involvement in diseaseGenetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
-
Sequence similaritiesBelongs to the CD24 family.
-
Post-translational
modificationsExtensively O-glycosylated. -
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 100133941 Human
- Omim: 600074 Human
- SwissProt: P25063 Human
- Unigene: 644105 Human
- Unigene: 721443 Human
-
Alternative names
- CD 24 antibody
- CD24 antibody
- CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
see all
Images
-
![Flow Cytometry / FACS - CD24 antibody [SN3] (ab30350)](http://a.abcam.com/ps/products/30/ab30350/Images/cd24fitc.jpg)
Flow Cytometry / FACS - CD24 antibody [SN3] (ab30350)Staining of human peripheral blood granulocytes with SN3 mouse monoclonal anti-human CD24 FITC conjugated (ab30350) -
![Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [SN3] (FITC) (ab30350)](http://a.abcam.com/ps/products/30/ab30350/Images/CD24-Primary-antibodies-ab30350-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [SN3] (FITC) (ab30350)This image is courtesy of an Abreview submitted by Dr Jennifer Dembinskiab30350 staining CD24 in human pancreatic adenocarcinoma cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and blocked with 3% BSA for 30 minutes at 24°C. The primary antibody (1/200 in 1%BSA in PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary.
References
This product has been referenced in:
- Wang L et al. Gemcitabine treatment induces endoplasmic reticular (ER) stress and subsequently upregulates urokinase plasminogen activator (uPA) to block mitochondrial-dependent apoptosis in Panc-1 cancer stem-like cells (CSCs). PLoS One 12:e0184110 (2017). Read more (PubMed: 28854261) »
- Leung CO et al. Sox9 confers stemness properties in hepatocellular carcinoma through Frizzled-7 mediated Wnt/ß-catenin signaling. Oncotarget 7:29371-86 (2016). Human . Read more (PubMed: 27105493) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"