Customer reviews and FAQs

Overview

  • Product nameAnti-CD24 antibody [SN3] (FITC)
    See all CD24 primary antibodies
  • Description
    Mouse monoclonal [SN3] to CD24 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Glycoproteins purified from human NALM-1 cell line

Applications

Our Abpromise guarantee covers the use of ab30350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Based on customer feedback, the use of a secondary antibody may be necessary

Target

  • FunctionModulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
  • Tissue specificityB-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
  • Involvement in diseaseGenetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
  • Sequence similaritiesBelongs to the CD24 family.
  • Post-translational
    modifications    Extensively O-glycosylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 24 antibody
      • CD24 antibody
      • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
      • CD24 antigen antibody
      • CD24 molecule antibody
      • CD24_HUMAN antibody
      • CD24A antibody
      • FLJ22950 antibody
      • FLJ43543 antibody
      • GPI linked surface mucin antibody
      • Heat stable antigen antibody
      • HSA antibody
      • MGC75043 antibody
      • Nectadrin antibody
      • Signal transducer CD24 antibody
      • Small cell lung carcinoma cluster 4 antigen antibody
      see all

    Anti-CD24 antibody [SN3] (FITC) images

    • Flow Cytometry / FACS - CD24 antibody [SN3] (ab30350)
      Flow Cytometry / FACS - CD24 antibody [SN3] (ab30350)
      Staining of human peripheral blood granulocytes with SN3 mouse monoclonal anti-human CD24 FITC conjugated (ab30350)
    • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [SN3] (FITC) (ab30350)
      Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [SN3] (FITC) (ab30350)This image is courtesy of an Abreview submitted by Dr Jennifer Dembinski
      ab30350 staining CD24 in human pancreatic adenocarcinoma cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and blocked with 3% BSA for 30 minutes at 24°C. The primary antibody (1/200 in 1%BSA in PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary.

      See Abreview

    References for Anti-CD24 antibody [SN3] (FITC) (ab30350)

    This product has been referenced in:
    • Stempin S  et al. Morphological and molecular characterization of the human breast epithelial cell line M13SV1 and its tumorigenic derivatives M13SV1-R2-2 and M13SV1-R2-N1. Cancer Cell Int 15:110 (2015). ICC/IF ; Human . Read more (PubMed: 26612978) »
    • Sims-Mourtada J  et al. Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds. Int J Nanomedicine 9:995-1003 (2014). Flow Cyt . Read more (PubMed: 24570583) »

    See all 8 Publications for this product

    Publishing research using ab30350? Please let us know so that we can cite the reference in this datasheet.

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    4 Abreviews or Q&A

    10
    Votes: Highest First

    Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti...

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    Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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    I have used ab30350 in the past and it worked well in ICC. The current lot, GR30681-1 does not stain cell as expected. The previous lot stained the plasma membrane but the current lot stains the nucleus, which is not where CD24 is expressed. I am stain...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab30350 with the order number 946525. To check the status of t...

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD24 antibody [SN3] (FITC)

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Pancreatic adenocarcinoma)
    Specification Pancreatic adenocarcinoma
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
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    abreview image
    Username

    Dr. Jennifer Dembinski

    Verified customer

    Submitted Nov 30 2009

    We are interested in doing some double immunofluorescent staining of cells for CD44 and CD24. As they are both membrane located we need to go the Fluorescent route. The cells are human and fresh from biopsy samples. There is also a possibility of apply...

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    Thank you for your enquiry. We do not have a CD44 antibody that is directly flurophore labelled that has been shown to work by BOTH IHC-Fr and IHC-P. However, we do have the following unconjugated antibody shown to work by both applications: ab2...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"