Overview

  • Product name
    Anti-CD24 antibody [SN3] (FITC)
    See all CD24 primary antibodies
  • Description
    Mouse monoclonal [SN3] to CD24 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Glycoproteins purified from human NALM-1 cell line

Applications

Our Abpromise guarantee covers the use of ab30350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells.

1/1 - 1/10. Use 10µl of the above dilution for 106 cells or 100ul whole blood.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Based on customer feedback, the use of a secondary antibody may be necessary

Target

  • Function
    Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
  • Tissue specificity
    B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
  • Involvement in disease
    Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
  • Sequence similarities
    Belongs to the CD24 family.
  • Post-translational
    modifications
    Extensively O-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 24 antibody
    • CD24 antibody
    • CD24 antigen (small cell lung carcinoma cluster 4 antigen) antibody
    • CD24 antigen antibody
    • CD24 molecule antibody
    • CD24_HUMAN antibody
    • CD24A antibody
    • FLJ22950 antibody
    • FLJ43543 antibody
    • GPI linked surface mucin antibody
    • Heat stable antigen antibody
    • HSA antibody
    • MGC75043 antibody
    • Nectadrin antibody
    • Signal transducer CD24 antibody
    • Small cell lung carcinoma cluster 4 antigen antibody
    see all

Images

  • Staining of human peripheral blood granulocytes with SN3 mouse monoclonal anti-human CD24 FITC conjugated (ab30350)
  • ab30350 staining CD24 in human pancreatic adenocarcinoma cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and blocked with 3% BSA for 30 minutes at 24°C. The primary antibody (1/200 in 1%BSA in PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Leung CO  et al. Sox9 confers stemness properties in hepatocellular carcinoma through Frizzled-7 mediated Wnt/ß-catenin signaling. Oncotarget 7:29371-86 (2016). Human . Read more (PubMed: 27105493) »
  • Stempin S  et al. Morphological and molecular characterization of the human breast epithelial cell line M13SV1 and its tumorigenic derivatives M13SV1-R2-2 and M13SV1-R2-N1. Cancer Cell Int 15:110 (2015). ICC/IF ; Human . Read more (PubMed: 26612978) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab30350 with the order number 946525. To check the status of t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Pancreatic adenocarcinoma)
Specification
Pancreatic adenocarcinoma
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Jennifer Dembinski

Verified customer

Submitted Nov 30 2009

Thank you for your enquiry. We do not have a CD44 antibody that is directly flurophore labelled that has been shown to work by BOTH IHC-Fr and IHC-P. However, we do have the following unconjugated antibody shown to work by both applications: ab2...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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