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Western blot - CD26 antibody - Carboxyterminal end (ab28341)

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Product Name
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CD26 antibody - Carboxyterminal end
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See all CD26 antibodies (22)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CD26 - Carboxyterminal end
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Immunogen
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Synthetic peptide based on the C terminal end of the catalytic domain of human CD26.
(Peptide available as ab41396.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab28341 does not recognize other DPP family members.
We have a range of domain specific antibodies for this target. For a full list please see all CD26 antibodies
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/1000 (colorimetric) - 1/5000 (chemiluminescent). Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type II membrane protein. Also exists in a soluble form.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> DPPs Immunology >> Cell Type Markers >> CD >> Adhesion Immunology >> Cell Type Markers >> CD >> Non-lineage Immunology >> Adaptive Immunity >> T Cells >> CD Immunology >> Adaptive Immunity >> B Cells >> CD
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Relevance
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CD26 [also known as dipeptidyl peptidase IV (DPP IV), adenosine deaminase (ADA) binding protein] is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Purification notes
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The antibody has been peptide-affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD26 antibody - Carboxyterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD26 antibody - Carboxyterminal end images, references, products related to ab28341 and other tools.
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CD26 antibody - Carboxyterminal end images:
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Western blot - CD26 antibody - Carboxyterminal end (ab28341)
All lanes : CD26 antibody - Carboxyterminal end (ab28341) at 1/1000 dilution
Lane 1 : Recombinant human DPP4/CD26 at 0.08 µg Lane 2 : Recombinant human DPP4/CD26 at 0.04 µg Lane 3 : Recombinant human DPP4/CD26 at 0.02 µg Lane 4 : Recombinant human DPP4/CD26 at 0.01 µg
Predicted band size : 88 kDa Observed band size : 114 kDa (why is the actual band size different from the predicted?)
Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels.
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References for CD26 antibody - Carboxyterminal end (ab28341)
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This product has been used in:
Stechly L et al. Galectin-4-regulated delivery of glycoproteins to the brush border membrane of enterocyte-like cells. Traffic 10:438-50 (2009). WB. PubMed: 19192249
If you publish research using ab28341 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD26
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CD26 antibody - Carboxyterminal end - more information
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Customer reviews (feedback) regarding CD26 antibody - Carboxyterminal end |
Customer FAQs regarding CD26 antibody - Carboxyterminal end |
Protocols for CD26 antibody - Carboxyterminal end |
Price and availability of products related to CD26 antibody - Carboxyterminal end |
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CD26 antibody - Carboxyterminal end - related products:
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CD26 antibody - Carboxyterminal end - other tools:
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Contact Abcam with a Technical Enquiry about CD26 antibody - Carboxyterminal end |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"