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Anti-CD26 antibody - Carboxyterminal end (ab28341)

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Overview

Product name

Anti-CD26 antibody - Carboxyterminal end
See all CD26 products (29) ...

Description

Rabbit polyclonal to CD26 - Carboxyterminal end

Specificity

ab28341 does not recognize other DPP family members.

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide based on the C terminal end of the catalytic domain of human CD26.

(Peptide available as ab41396.)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody has been peptide-affinity purified.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - CD26 antibody - Carboxyterminal end (ab28341)Western blot - CD26 antibody - Carboxyterminal end (ab28341) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/5000.Dete...Read more →

    WB: 1/1000 - 1/5000.Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa).(1/1000 (colorimetric) - 1/5000 (chemiluminescent). EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.)

Target

Function

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.

Tissue specificity

Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.

Sequence similarities

Belongs to the peptidase S9B family. DPPIV subfamily.

Domain

The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity.

Post-translational
modifications

The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation.

Cellular localization

Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid.

Target information above from: UniProt accessionP27487 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ADA-binding protein antibody
  • ADABP antibody
  • ADCP 2 antibody
  • ADCP-2 antibody
  • ADCP2 antibody
  • ADCP2 antibody
  • Adenosine deaminase complexing protein 2 antibody
  • Adenosine deaminase complexing protein 2 antibody
  • CD 26 antibody
  • CD26 antibody
  • CD26 antigen 3 antibody
  • Dipeptidyl peptidase 4 antibody
  • Dipeptidyl peptidase 4 soluble form antibody
  • Dipeptidyl peptidase iv antibody
  • Dipeptidyl peptidase iv antibody
  • Dipeptidyl peptidase IV membrane form antibody
  • Dipeptidyl peptidase IV soluble form antibody
  • Dipeptidylpeptidase 4 antibody
  • Dipeptidylpeptidase IV antibody
  • DPP 4 antibody
  • DPP IV antibody
  • DPP IV estoenzyme antibody
  • DPP4 antibody
  • DPP4 antibody
  • DPP4_HUMAN antibody
  • DPPIV antibody
  • Intestinal dipeptidyl peptidase antibody
  • Intestinal dipeptidyl peptidase antibody
  • T cell activation antigen CD26 antibody
  • T cell activation antigen CD26 antibody
  • T-cell activation antigen CD26 antibody
  • TP 103 antibody
  • TP103 antibody
see all

Anti-CD26 antibody - Carboxyterminal end images:

  Western blot - CD26 antibody - Carboxyterminal end (ab28341)

Western blot - CD26 antibody - Carboxyterminal end (ab28341)

All lanes : Anti-CD26 antibody - Carboxyterminal end (ab28341) at 1/1000 dilution

Lane 1 : Recombinant human DPP4/CD26 at 0.08 µg
Lane 2 : Recombinant human DPP4/CD26 at 0.04 µg
Lane 3 : Recombinant human DPP4/CD26 at 0.02 µg
Lane 4 : Recombinant human DPP4/CD26 at 0.01 µg


Predicted band size : 88 kDa
Observed band size : 114 kDa (why is the actual band size different from the predicted?)


Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels.

References for Anti-CD26 antibody - Carboxyterminal end (ab28341)

This product has been referenced in:

  • Conde-Vancells Jet al. Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. Proteomics Clin Appl 4:416-425 (2010). WB; Rat.Read more (PubMed: 20535238) »
  • Stechly Let al. Galectin-4-regulated delivery of glycoproteins to the brush border membrane of enterocyte-like cells. Traffic 10:438-50 (2009). WB.Read more (PubMed: 19192249) »

See all 2 publications for this product

Publishing research using ab28341? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"