Our Abpromise guarantee covers the use of ab70103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 29 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • FunctionReceptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificityFound in most T-lymphocytes.
  • Sequence similaritiesContains 3 TNFR-Cys repeats.
  • Post-translational
    Phosphorylated and O-glycosylated.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 27 antibody
    • CD27 antibody
    • CD27 antigen antibody
    • CD27 molecule antibody
    • CD27_HUMAN antibody
    • CD27L receptor antibody
    • LPFS2 antibody
    • MGC20393 antibody
    • OTTHUMP00000238557 antibody
    • S152 antibody
    • T cell activation antigen CD27 antibody
    • T cell antivation antigen S152 antibody
    • T-cell activation antigen CD27 antibody
    • T14 antibody
    • TNFRSF 7 antibody
    • TNFRSF7 antibody
    • TNFSF7 antibody
    • Tp 55 antibody
    • Tp55 antibody
    • Tumor necrosis factor receptor superfamily member 7 antibody
    see all

Anti-CD27 antibody images

  • All lanes : Anti-CD27 antibody (ab70103) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab70103 staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70103, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD27 antibody (ab70103)

This product has been referenced in:
  • Gramlich OW  et al. Enhanced insight into the autoimmune component of glaucoma: IgG autoantibody accumulation and pro-inflammatory conditions in human glaucomatous retina. PLoS One 8:e57557 (2013). Human . Read more (PubMed: 23451242) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

Submitted Dec 16 2010