Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)
Key features and details
- Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
See all CD27 primary antibodies -
Description
Armenian hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free -
Host species
Armenian hamster -
Specificity
When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- BALB/c mouse splenocytes.
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General notes
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS
Aqueous buffer, no sodium azide. -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Functional grade purified. -
Clonality
Monoclonal -
Clone number
LG.3A10 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.25µg for 105-8 cells.
In a final volume of 100 μL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 0.25µg for 105-8 cells. In a final volume of 100 μL. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 939 Human
- Entrez Gene: 21940 Mouse
- Omim: 186711 Human
- SwissProt: P26842 Human
- SwissProt: P41272 Mouse
- Unigene: 355307 Human
- Unigene: 367714 Mouse
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Alternative names
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab185776 has not yet been referenced specifically in any publications.