Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free (ab185776)

Overview

  • Product name
    Anti-CD27 antibody [LG.3A10] - Low endotoxin, Azide free
    See all CD27 primary antibodies
  • Description
    Armenian Hamster monoclonal [LG.3A10] to CD27 - Low endotoxin, Azide free
  • Host species
    Armenian hamster
  • Specificity
    When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction.
  • Tested applications
    Suitable for: IHC-Fr, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • BALB/c mouse splenocytes.
  • General notes

    Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.

Properties

Applications

Our Abpromise guarantee covers the use of ab185776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use 0.25µg for 105-8 cells.

In a final volume of 100 μL.

ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
  • Tissue specificity
    Found in most T-lymphocytes.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Post-translational
    modifications
    Phosphorylated and O-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 27 antibody
    • CD27 antibody
    • CD27 antigen antibody
    • CD27 molecule antibody
    • CD27_HUMAN antibody
    • CD27L receptor antibody
    • LPFS2 antibody
    • MGC20393 antibody
    • OTTHUMP00000238557 antibody
    • S152 antibody
    • T cell activation antigen CD27 antibody
    • T cell antivation antigen S152 antibody
    • T-cell activation antigen CD27 antibody
    • T14 antibody
    • TNFRSF 7 antibody
    • TNFRSF7 antibody
    • TNFSF7 antibody
    • Tp 55 antibody
    • Tp55 antibody
    • Tumor necrosis factor receptor superfamily member 7 antibody
    see all

Images

  • Flow cytometric analysis of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.125 µg of ab185776 followed by Anti-Armenian Hamster IgG PE. Total viable cells were analyzed.

References

ab185776 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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