Anti-CD27 antibody [MM0165-7H21] (ab89258)
Key features and details
- Mouse monoclonal [MM0165-7H21] to CD27
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD27 antibody [MM0165-7H21]
See all CD27 primary antibodies -
Description
Mouse monoclonal [MM0165-7H21] to CD27 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant Human CD27 protein (also known as TNFRSF7 extracellular domain).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute the antibody with 200 µl sterile PBS and the final concentration is 500 µg/ml. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography -
Clonality
Monoclonal -
Clone number
MM0165-7H21 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa. |
Target
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Function
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
Tissue specificity
Found in most T-lymphocytes. -
Sequence similarities
Contains 3 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 939 Human
- Omim: 186711 Human
- SwissProt: P26842 Human
- Unigene: 355307 Human
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Alternative names
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
Images
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Anti-CD27 antibody [MM0165-7H21] (ab89258) at 1/1000 dilution (Mouse anti human TNFRSF7) + Human spleen tissues lysate
Predicted band size: 29 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab89258 has not yet been referenced specifically in any publications.