Overview

  • Product name
  • Description
    Rabbit polyclonal to CD272
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 - 164 of Human CD272 (NP_001078826).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, MOLT4 and Raji cell lysates IHC-P: MDAMB468 xenograft

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B and T lymphocyte associated protein antibody
    • B and T lymphocyte attenuator antibody
    • B and T lymphocyte associated antibody
    • B- and T-lymphocyte attenuator antibody
    • B- and T-lymphocyte-associated protein antibody
    • BTLA antibody
    • BTLA_HUMAN antibody
    • BTLA1 antibody
    • CD272 antibody
    • CD272 antigen antibody
    • FLJ16065 antibody
    • MGC129743 antibody
    see all

Images

  • All lanes : Anti-CD272 antibody (ab96560) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysates
    Lane 2 : Raji whole cell lysates

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
  • ab96560, at a 1/100 dilution, staining CD272 in paraffin embedded MDAMB468 xenograft by Immunohistochemical analysis.

References

This product has been referenced in:
  • Ramsay AG  et al. Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood 120:1412-21 (2012). IHC ; Human . Read more (PubMed: 22547582) »

See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab96560.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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