The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 105 cells. Use at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Flow cytometry staining of BALB/c splenocytes with Anti-Mouse CD3e FITC and 0.25 µg of Golden Syrian Hamster IgG Isotype Control APC (left) or 0.25 µg of ab95559 (right). Total viable cells were used for analysis.
References for Anti-CD28 antibody [37.51] (Allophycocyanin) (ab95559)