Overview

  • Product nameAnti-CD28 antibody [37.51] (Allophycocyanin)
    See all CD28 primary antibodies
  • Description
    Syrian Hamster monoclonal [37.51] to CD28 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    CHO cell line CN5.1 transfected with a murine cDNA (J. Immunology; 149 (2); 380; PubMed:1320641)

  • Positive control
    • BALB/c splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105 cells. Use at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. Note: It has been observed that increased incubations times (30-45 minutes) with the CD28 antibody can enhance the staining obtained.

Target

  • FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

Anti-CD28 antibody [37.51] (Allophycocyanin) images

  • Flow cytometry staining of BALB/c splenocytes with Anti-Mouse CD3e FITC and 0.25 µg of Golden Syrian Hamster IgG Isotype Control APC (left) or 0.25 µg of ab95559 (right). Total viable cells were used for analysis.

References for Anti-CD28 antibody [37.51] (Allophycocyanin) (ab95559)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"