Overview

  • Product name
  • Description
    Goat polyclonal to CD28
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    SQQLQVYSKTGFNCD

    , corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1).

  • Positive control
    • Human lymph node and tonsil lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab113358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity
    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

Images

  • ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

     

  • ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

  • Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.

    Note: The observed molecular weight corresponds to the glycosylated form.

References

ab113358 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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