Overview

  • Product nameAnti-CD28 antibody
    See all CD28 primary antibodies
  • Description
    Goat polyclonal to CD28
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    SQQLQVYSKTGFNCD

    , corresponding to internal sequence amino acids 73-87 of Human CD28 (NP_006130.1).

  • Positive control
    • Human lymph node and tonsil lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab113358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

Anti-CD28 antibody images

  • ab113358 (2µg/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

     

  • ab113358 negative control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.

  • Anti-CD28 antibody (ab113358) at 0.3 µg/ml + Human tonsil lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.

    Note: The observed molecular weight corresponds to the glycosylated form.

References for Anti-CD28 antibody (ab113358)

ab113358 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113358.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"