PerCP Anti-CD28 antibody [CD28.2] (ab88034)
Key features and details
- PerCP Mouse monoclonal [CD28.2] to CD28
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PerCP Anti-CD28 antibody [CD28.2]
See all CD28 primary antibodies -
Description
PerCP Mouse monoclonal [CD28.2] to CD28 -
Host species
Mouse -
Conjugation
PerCP. Ex: 482nm, Em: 675nm -
Specificity
Reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to CD28. DC28.1.3.3 hybridoma cells
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Positive control
- Human blood cells
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General notes
The purified antibody (>95% by SDS-PAGE) is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% BSA (high-grade, protease free) -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
CD28.2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
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Notes |
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Flow Cyt
Use 10µl for 106 cells. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab88034 has been referenced in 7 publications.
- Scharschmidt E et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780
- Jeong SH et al. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol 78:6995-7003 (2004). PubMed: 15194776
- Marti F et al. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol 166:197-206 (2001). PubMed: 11123293
- Galea-Lauri J et al. Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol 163:62-70 (1999). PubMed: 10384100
- Tazi A et al. Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol 163:3511-5 (1999). PubMed: 10477625
- Nunès J et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol 5:311-5 (1993). PubMed: 8385476
- Nunes J et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J 293 ( Pt 3):835-42 (1993). PubMed: 8394695