Anti-CD28 antibody [JJ319] (Biotin) (ab95561)

Overview

  • Product nameAnti-CD28 antibody [JJ319] (Biotin)
    See all CD28 primary antibodies
  • Description
    Mouse monoclonal [JJ319] to CD28 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat CD28 transfected Mouse A20JB lymphoma

  • Positive control
    • Rat splenocytes

Properties

Applications

Our Abpromise guarantee covers the use of ab95561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 105 cells. Use at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.



ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

Anti-CD28 antibody [JJ319] (Biotin) images

  • Flow cytometry staining of rat splenocytes with Anti-Rat CD5 FITC and 0.25 µg of Mouse IgG1 ? Isotype Control Biotin (left) or 0.5 µg of ab95561 (right) followed by Streptavidin PE. Total viable cells were used for analysis.

References for Anti-CD28 antibody [JJ319] (Biotin) (ab95561)

ab95561 has not yet been referenced specifically in any publications.

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