Anti-CD28 antibody [PV-1] (ab25234)
Key features and details
- Armenian hamster monoclonal [PV-1] to CD28
- Suitable for: IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
-
Product name
Anti-CD28 antibody [PV-1]
See all CD28 primary antibodies -
Description
Armenian hamster monoclonal [PV-1] to CD28 -
Host species
Armenian hamster -
Tested applications
Suitable for: IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD28. C57BL/6N mouse T cell lymphoma EL4 cell line
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PV-1 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use at an assay dependent concentration.
|
|
Flow Cyt |
Use at an assay dependent concentration.
In vitro costimulation of T cells and NK cells. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
Functional Studies |
Use at an assay dependent concentration.
In vitro costimulation of T cells and NK cells. |
Notes |
---|
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. In vitro costimulation of T cells and NK cells. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. In vitro costimulation of T cells and NK cells. |
Target
-
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12487 Mouse
- SwissProt: P31041 Mouse
- Unigene: 255003 Mouse
-
Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab25234 has been referenced in 6 publications.
- Bevington SL et al. Chromatin Priming Renders T Cell Tolerance-Associated Genes Sensitive to Activation below the Signaling Threshold for Immune Response Genes. Cell Rep 31:107748 (2020). PubMed: 32521273
- Wang J et al. PDL1 Fusion Protein Protects Against Experimental Cerebral Malaria via Repressing Over-Reactive CD8+ T Cell Responses. Front Immunol 9:3157 (2018). PubMed: 30693001
- Meyer Zu Horste G et al. Predicting the Response to Intravenous Immunoglobulins in an Animal Model of Chronic Neuritis. PLoS One 11:e0164099 (2016). Flow Cyt . PubMed: 27711247
- Mascanfroni ID et al. Metabolic control of type 1 regulatory T cell differentiation by AHR and HIF1-a. Nat Med 21:638-46 (2015). PubMed: 26005855
- Fischer N et al. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun 6:6113 (2015). PubMed: 25672245
- Quintana FJ et al. Aiolos promotes TH17 differentiation by directly silencing Il2 expression. Nat Immunol 13:770-7 (2012). PubMed: 22751139