Overview

  • Product nameAnti-CD299 antibody
    See all CD299 primary antibodies
  • Description
    Rabbit polyclonal to CD299
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, African Green Monkey
  • Immunogen

    A region within synthetic peptide: SKVPSSLSQE QSEQDAIYQN LTQLKAAVGE LSEKSKLQEI YQELTQLKAA, corresponding to N terminal amino acids 73-122 of Human CD299

  • Positive control
    • HepG2 cell lysate; human kidney tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab58603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionProbable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
  • Tissue specificityPredominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 4, member M antibody
    • C-type lectin domain family 4 member M antibody
    • CD209 antigen like antibody
    • CD209 antigen like protein 1 antibody
    • CD209 antigen-like protein 1 antibody
    • CD209L antibody
    • CD209L1 antibody
    • CD299 antibody
    • CD299 antigen antibody
    • CLC4M_HUMAN antibody
    • CLEC4M antibody
    • DC SIGN related protein antibody
    • DC SIGN2 antibody
    • DC SIGNR antibody
    • DC-SIGN-related protein antibody
    • DC-SIGN2 antibody
    • DC-SIGNR antibody
    • DCSIGN related protein antibody
    • DCSIGNR antibody
    • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
    • HP10347 antibody
    • L SIGN antibody
    • L-SIGN antibody
    • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
    • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
    • LSIGN antibody
    • Mannose binding C type lectin DC SIGNR antibody
    • MGC129964 antibody
    • MGC47866 antibody
    see all

Anti-CD299 antibody images

  • Anti-CD299 antibody (ab58603) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD299 with ab58603 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • ICC/IF image of ab58603 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58603, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD299 antibody (ab58603)

This product has been referenced in:
  • Martinez MG  et al. Utilization of human DC-SIGN and L-SIGN for entry and infection of host cells by the New World arenavirus, Junín virus. Biochem Biophys Res Commun 441:612-7 (2013). Human . Read more (PubMed: 24183720) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"